Black Seed Oil comes from the seeds of Nigella sativa and contains a bioactive compound called thymoquinone. This oil has been used in traditional medicine for centuries and is now supported by research for its anti-inflammatory, anticancer, antioxidant, and immunomodulatory effects.

In Protocol 2, 3 tablespoons of Black Seed Oil are taken during the OMAD meal to:

  • Inhibit tumor growth and angiogenesis
  • Enhance immune response and reduce inflammation
  • Support healing of the gut lining post-radiation
  • Provide healthy fats to assist with supplement absorption and satiety during fasting

🔍 Black Seed Oil – OMAD Phase Summary

✅ Best Timing:

  • 2:30–4:30 PM during the OMAD + Second Wave Phase
  • Take with the main meal to enhance absorption of fat-soluble supplements and aid gut recovery
  • Do not take near the oxidative phase (B17, MB, radiation), as it is a strong antioxidant

💊 Recommended Dose:

  • 3 tablespoons (approx. 45 mL) per day, split across or taken with OMAD
  • Cold-pressed, unrefined oil is ideal for maximum bioactivity
  • Start with 1 tbsp/day and increase to 3 tbsp over time to avoid GI distress

⏳ Active Duration in Body:

  • Effects begin within 1–2 hours
  • Anti-inflammatory and metabolic effects last 6–12 hours
  • Consistent daily use promotes gut repair, immune regulation, and tumor suppression

🔁 Redundancy With:

  • Some antioxidant and immune overlap with Curcumin, Andrographis, and Diosmetin
  • Unique combination of lipid-based immune modulation + thymoquinone-specific cancer effects
  • Also enhances absorption of fat-soluble compounds like Ursolic Acid, Vitamin D/K2, and Honokiol

📉 Pathways Inhibited or Affected:

  • NF-κB inhibition – reduces chronic inflammation that fuels tumor progression
  • STAT3 inhibition – limits immune evasion and metastasis
  • PI3K/Akt/mTOR inhibition – suppresses growth and proliferation
  • VEGF downregulation – blocks angiogenesis
  • Thymoquinone triggers apoptosis and inhibits tumor migration
  • Promotes gut mucosal healing, beneficial microbiota, and tight junction support

🔒 Final Summary

Black Seed Oil is one of the most comprehensive natural allies against cancer. In Protocol 2, it is taken at 3 tablespoons during the OMAD phase to inhibit cancer growth, reduce inflammation, promote gut repair, and support the immune system.

Its high fat content also supports satiety, metabolic flexibility, and the absorption of key anti-cancer supplements—making it a vital base of the afternoon recovery strategy.

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