Pterostilbene is a natural compound found in blueberries and grapes. It’s a methylated analog of Resveratrol, offering better bioavailability, longer duration of action, and a similar range of benefits—especially for anti-cancer effects, DNA protection, and NAD+ preservation.

In Protocol 2, Trans-Pterostilbene is used during the Antioxidant Wave Phase to:

  • Support SIRT1 activation and NAD+ recycling
  • Protect healthy cells from damage after oxidative therapies
  • Inhibit inflammation, angiogenesis, and key cancer survival pathways

🔍 Trans-Pterostilbene – Protocol 2 Summary

✅ Best Timing:

  • 12:30 PM during the Antioxidant Wave Phase
  • Pairs well with Resveratrol, Quercetin, EGCG, and Fisetin
  • Do not take within 6–8 hours of oxidative-phase supplements (B17, Artemisinin, MB, radiation)

💊 Recommended Dose:

  • 200 mg once daily, preferably in the trans- form (more bioactive)
  • Take with food or healthy fat to enhance absorption
  • May be stacked with Resveratrol for synergy but monitor for cumulative blood-thinning effects

⏳ Active Duration in Body:

  • Highly bioavailable; peak effects within 1–2 hours
  • Active in the body for 8–12+ hours, longer than Resveratrol
  • Daily use builds mitochondrial and epigenetic resilience

🔁 Redundancy With:

  • Similar mechanism to Resveratrol (both activate SIRT1, reduce inflammation, protect DNA)
  • Longer-lasting than Resveratrol but complementary—can be stacked or alternated
  • Avoid during oxidative kill windows due to antioxidant ROS-suppressing effects

📉 Pathways Inhibited or Affected:

  • NF-κB – blocks inflammation and tumor survival
  • PI3K/Akt/mTOR – suppresses growth and insulin resistance
  • SIRT1 activation – supports DNA repair and longevity pathways
  • Angiogenesis inhibition – reduces blood supply to tumors
  • Supports NAD+ preservation and mitochondrial health

🔒 Final Summary

Trans-Pterostilbene is a long-lasting, high-impact antioxidant used at 200 mg during the 12:30 PM Antioxidant Wave Phase in Protocol 2. It mimics and enhances Resveratrol’s benefits while lasting longer and better resisting breakdown in the body.

Its role is to protect DNA, support NAD+, activate longevity genes, and reduce cancer-promoting inflammation—making it a key component of post-oxidative recovery.

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